Cloned (Comment) | Organism |
---|---|
isolation of a cDNA encoding an enzyme by functional complementation of the Escherichia coli mutant plsC with an immature embryo cDNA library of oilseed rape. Transformation of the acyltransferase-deficient Escherichia coli strain JC201 with the cDNA sequence BAT2 alleviates the temperature-sensitive phenotype of the plsC mutant and confers a palmitoyl-CoA-preferring enzyme activity to membrane fractions. Mapping of the BAT2 genes and sequence analysis of the BAT2 cDNA clone | Brassica napus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | mature protein of 32000 Da processed from the precursor protein of 38000 Da | Brassica napus | 9507 | - |
membrane | - |
Brassica napus | 16020 | - |
plastid | - |
Brassica napus | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
mature protein, processing of a BAT2 precursor protein, chloroplast-import experiments | Brassica napus |
38000 | - |
predicted, protein encoded by the BAT2 cDNA | Brassica napus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Brassica napus | the enzyme controls phosphatidic acid biosynthesis in the plastid | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the BAT2 gene is expressed in a ubiquitous manner in all tissues, including nonphotosynthetic tissues where plastids are present | Brassica napus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
Brassica napus | CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
additional information | the enzyme controls phosphatidic acid biosynthesis in the plastid | Brassica napus | ? | - |
? | |
oleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | assayed with BAT2 protein | Brassica napus | CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-ACP + 1-acyl-sn-glycerol 3-phosphate | - |
Brassica napus | ACP + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | assayed with BAT2 protein, palmitoyl-CoA is the preferred substrate | Brassica napus | CoA + 1-oleoyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Brassica napus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Brassica napus |